Comparison of nitrifying bacteria and denitrifying bacteria

Comparison of nitrifying bacteria and denitrifying bacteria

It's written for fun, just take a look at it...

Some things you need to know:

1. Nitrite is produced by bacteria! And it can only be produced by bacteria! These bacteria are collectively called nitrosative bacteria, which are very annoying. There will always be some ammonia nitrogen or amino acids in the water, and it just so happens that the algae in this pool of water is relatively rich, and it happens that the sun is brighter today, and it is unfortunate that some nitrosative bacteria live in your pool, and it is even more unfortunate that you just happen to be blooming. Take off the oxygenator. Well, ammonia nitrogen and amino acids are very energy-rich things. Once they react with oxygen (produced by photosynthesis and produced by the oxygen generator), your nitrite is waiting to rise!

You will definitely ask, since this is the case, shouldn’t it be enough for me to get rid of all the nitrosative bacteria? But unfortunately, I don't think it's possible. Because in fact: as long as the aerobic organisms that can use ammonia nitrogen have a chance to become nitrosating bacteria, I don't know how to kill them at all. They are just like the people, they are inexhaustible, and they are born again when the spring breeze blows.

2. Both nitrifying bacteria and denitrifying bacteria refer to it in general. In fact, there are no separate comrades called nitrifying bacteria and denitrifying bacteria in microorganisms.

3. Ammonia nitrogen can be changed into nitrite, nitrite can be converted into nitrate, nitrite can be changed into nitrogen, nitrogen is very good and environmentally friendly.

4. Ammonia nitrogen has more energy, nitrite has a little energy, and nitrate has no energy. But nitrite and nitrate are strong oxidants, so (ammonium) nitrate (ammonia) can be used to make explosives, very yellow and violent.

The following comparison begins:

1. What to eat?

Nitrifying bacteria are bitter children, they specialize in eating what others don’t want to eat, or the leftover nitrite. Nitrite is like a bone, hard to chew and not nutritious, so generally it grows slowly and reproduces. Generation is even more difficult. The most embarrassing thing is that you want to give him something good to eat, but he still refuses to admit it, and tells you: "I'm sorry, I'm used to it, and the taste can't be changed. You can keep it for yourself. I'll still eat my bones." Go." Do you mean cheap?

The denitrifying bacteria were born in a rich family, they are used to delicacies from mountains and seas, and they like to eat glucose with the highest nutrition and taste* the most. That thing is long! Raise people! Therefore, the denitrifying bacteria are well maintained, handsome, and all the girls throw themselves into their arms. In one day's work, the house is full of children and grandchildren.

2. What to smoke?

Smoke... what? Smoking? Yes, like smoking, both bacteria share these proclivities. Unlike humans, they smoke to grow their bodies and burn their life flames. The nitrifying bacteria pumps oxygen, which is what the general public likes to smoke. Hello everyone, it is really good. The price is cheap and the quantity is plentiful. We have been pumping it all the time. As a representative of poor people, nitrifying bacteria are certainly no exception. However, oxygen is often a scarce item. Once it is out of stock, all the fish, shrimps and bacteria in the pool will search for cigarette butts all over the street, which is really embarrassing.

The denitrifying bacteria said: "Actually, don't look at me, I also like to be close to life and close to the people. I also like to smoke oxygen, but it is always out of stock. Is it difficult for me to grab cigarette butts from civilians? That would kill my identity. Absolutely not. So, I smoke private goods, most people can’t stand it, it’s called opium. Hehe, in fact, it has another name, called nitrite. Don’t look at this cheap thing. Come on, in fact, nitrite is the most exciting when it is lit, like burning explosives (囧...), haha, I don’t tell the average person.” (Denitrifying bacteria, in fact, there is something you still have I don't know, nitrates are twice as powerful, but I guess you can't get them in the water, so you don't know.)

3. Hygiene is a big problem

"Speaking of civilization and hygiene" is about personal hygiene habits. Nitrifying bacteria are not very good. After nitrite is eaten by it, it will turn into nitrate, and it will be thrown away. The place is accumulating more and more, making it uncomfortable to choke on itself, making the appetite worse and worse, the speed of eating will become slower and slower, and the growth will also slow down.

The denitrifying bacteria is the pacesetter and model of hygiene in the bacterial world. It smokes nitrate and nitrite, eats glucose, and spits out pure and environmentally friendly nitrogen and carbon dioxide. Not only that, but it also pays attention to Pack up these rubbish and throw them into the trash can (drain the water body), the place where you live will always be clean, so it can always maintain a good appetite, eat as much as you like, no matter how fast you eat, you won’t have indigestion* .

Okay, let's be serious

Although denitrifying bacteria have many advantages compared with nitrifying bacteria, traditional denitrifying bacteria also have their own fatal shortcomings.

1. Pampered

Denitrifiers are sensitive to changes in the environment. If the temperature becomes too fast, it will die; if the moisture decreases too fast, it will die; if the pH changes too fast, it will die; Quickly, it will die; if it is put in the water and there is nothing to eat, it will die too; in a word, it is less resistant to stress. So if you see which denitrifying bacteria on the market are water-based, you can basically judge that the product is garbage.

In contrast, nitrifying bacteria are more resistant to stress and are easier to preserve.

2. Easy to aerobic

Although denitrifying bacteria use nitrite and nitrite very quickly, if there is oxygen, it will consume oxygen first, and will use nitrate and nitrite when the oxygen consumption is almost the same, so in the aquaculture water It is easy to lose effect.

Nitrifying bacteria consume both oxygen and nitrite at any time, so if there is sufficient oxygenation, sometimes there is still some effect.

Based on the above situation, we list a table:

nitrifying bacteria denitrifying bacteria

Growth rate slow very fast

Degradation rate Slow and unstable, incomplete Fast and stable, complete

Nutritional requirements Low, nitrite and oxygen are sufficient Higher, must have sugar

Effect Bounce Easy Bounce No Bounce

Aerobic must be aerobic, the more oxygen the better, it is easy to consume oxygen, which is not conducive to the degradation of nitrite

Preservation Easy to Preserve Difficult to Preserve

Others When the nitrate is high, it will be difficult to grow. When the nitrate is high, the nitrite will increase first, and then decrease. Of course, the aquaculture water will rarely contain a large amount of nitrate.

This is a comprehensive comparison of the natural varieties of these two bacteria. Note that they are natural varieties. Natural varieties must be modified, screened and processed before they can become useful microbial preparations. So what improvements have these two products undergone so far? And how should we choose the products on the market? Let's talk about it next time.

Since natural strains have one or another shortcoming, it is difficult to be directly used for water quality regulation. Therefore, as a responsible manufacturer, it is necessary to produce truly effective products through a series of means such as screening, transformation, and process improvement as much as possible.

The improvement direction of nitrifying bacteria:

1. In addition to nitrite, is it possible to confirm that the ability of nitrifying bacteria to utilize other nutrients has been removed?

In fact, natural nitrifying bacteria can often not only use nitrite as an energy source, but also use other energy sources such as organic matter, sugar, and even ammonia nitrogen, because in a natural environment, bacteria can only use nitrite to survive and reproduce. Nitrite is very inefficient, and if you are too picky, it will be difficult to survive in a complex competitive environment. However, as a product that specifically degrades nitrous acid, if these capabilities are not shielded or removed, it will easily cause failure in actual use. You added nitrifying bacteria, and you originally expected it to eat nitrite, but it turned out to eat other more Nutritious things will betray your expectations.

2. Has the effect of product inhibition been removed?

Nitrifying bacteria can convert nitrite into nitrate, but the more the converted nitrate accumulates, the slower the reaction efficiency to it is, which is called the product inhibition effect. This effect may be eliminated through strict screening or genetic modification. The essence is to modify the structure of the nitrification enzyme. If you are interested, you can refer to the information on enzymology.

3. Under the premise of the above two guarantees, is there a fast enough growth or reaction speed?

Needless to say, continuous breeding is the key

4. Is the preservation process perfect? Is there a lot of bacteria?

Although the stress resistance of nitrifying bacteria is not bad, if the storage method is not appropriate, it will die quickly. It should be explained here that liquid products are absolutely not acceptable! Except for bacteria such as lactic acid bacteria that can survive in a highly acidic environment (you should also store it in the refrigerator), bacteria cannot be stored for long in liquid conditions! It must be dried and the moisture content should be below 15%!

Improvement direction of denitrifying bacteria:

1. Have you lost your dependence on oxygen?

Oxygen has a competitive inhibitory effect on the use of nitrite by denitrifying bacteria, so the denitrifying bacteria must first be improved or screened to break away from the dependence on oxygen. Whether there is oxygen or not, it exclusively uses nitrite or nitrate as the oxidant

2. Is the preservation process really perfect?

The preservation of denitrifying bacteria has always been a difficult point. Under severe dry processing conditions, denitrifying bacteria are easy to die in large numbers, which requires manufacturers to improve their unique preservation processes. Also, liquid packaging of denitrifying bacteria is absolutely impossible!

3. Is there a reasonable activation procedure?

Denitrifying bacteria are saprophytic fungi, and saprophytic bacteria generally need to be activated to exert their maximum effect. For responsible products, activation steps are indispensable.

Having said all that, if:

A nitrifying bacteria product, if it does not mention "obligate utilization of nitrite, removal of product inhibitory effect", or if it is an aqueous product

Denitrifying bacteria product, if it does not mention "insensitive to oxygen, advanced preservation technology", or is a water-based product, or has no activation step

Then *don't even consider these products! Whether the efficacy is true or not, at least it means that the manufacturer is not aware of these problems.

I read it well and reprinted it. We analyze the mistakes.